Chapter 5: Troubleshooting 99
Tube does not load, or
sample tube does not fit
snugly on SIT
Improper sample tube used Use Falcon brand 12 x 75-mm
polystyrene test tubes,
BD Trucount tubes, or BD FACS
7-color setup bead tubes. See
System Requirements on page 31.
Bal seal improperly installed
or worn
Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 80.
Defective or cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes. See System
Requirements on page 31.
Bal seal improperly installed
or worn
Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 80.
Adapter lever in incorrect
position for manual loading
Make sure the lever is in the
manual loading position. Modify
the cytometer appropriately. See
Changing to Manual Loading on
page 39.
Higher than expected
CVs for some (brightest)
populations during DNA
analysis
Possible pre-amp non-
linearity with very bright
signals
Set G
0
/G
1
diploid population peak
at channel 25,000 or less.
>20% difference
between Measured and
Reference laser power
Flow cell access door open Close the door completely.
BD FACSDiva software
not launching
Cytometer.dat file not found • Install saved cytometer.dat file,
or
• call BD Biosciences for
assistance.
Cytometer Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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