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9. TEMPERATURE SAFETY DEVICES ................................................................... 42
9.1 Over temperature protective device (class 1) ................................................................................... 42
9.2 Safety controller (temperature safety device class 3.1) .................................................................... 42
9.2.1 Setting the safety controller set point type .............................................................................. 43
9.2.2 Setting the safety controller set point ...................................................................................... 44
10. ALARM FUNCTIONS ........................................................................................... 46
10.1 Alarm functions overview .................................................................................................................. 46
10.2 Resetting the alarm messages ......................................................................................................... 46
10.3 Zero-voltage relay alarm output ........................................................................................................ 47
10.4 Safety controller temperature alarm .................................................................................................. 48
10.5 Temperature tolerance range alarm (high and low temperature) ..................................................... 48
10.6 Door open ......................................................................................................................................... 49
10.7 CO
2
tolerance range alarm (CO
2
over/under concentration) ............................................................ 50
10.8 CO
2
pressure too low ........................................................................................................................ 50
10.9 Temperature sensor failure ............................................................................................................... 51
10.10 Failure of CO
2
sensor ........................................................................................................................ 52
11. ERROR MESSAGES ............................................................................................ 52
12. REFERENCE MEASUREMENTS ........................................................................ 53
12.1 CO
2
reference measuring ................................................................................................................. 53
12.1.1 Measuring the CO
2
concentration indirectly via the pH of the cell medium ............................ 53
12.1.2 Measuring CO
2
directly via chemical indicator tubes ............................................................. 54
12.1.3 Measuring CO
2
directly with an electronic infrared measuring device ................................... 55
12.2 Temperature reference measurement .............................................................................................. 55
13. OPTIONS .............................................................................................................. 55
13.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559
left, 8012-0560 right) (option) ............................................................................................................ 55
13.2 Base on castors (option) ................................................................................................................... 56
13.3 Stacking adapter for direct thermal decoupled stacking (option) ...................................................... 56
13.1 Analog outputs for temperature and CO
2
(option) ............................................................................ 56
14. AVOIDING MICROBIAL CONTAMINATION........................................................ 57
14.1 Cells and media ................................................................................................................................ 57
14.2 Laboratory conditions / equipment around the incubator .................................................................. 57
14.3 Working and behavior in the lab ....................................................................................................... 57
14.4 Chamber design and equipment of the CO
2
incubator C 170 .......................................................... 58
14.5 Handling the CO
2
incubator C 170 .................................................................................................... 59
15. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .... 60
15.1 Cleaning ............................................................................................................................................ 60
15.2 Decontamination / chemical disinfection of the CO
2
incubator ......................................................... 61
15.3 Disinfection of the CO
2
sensor .......................................................................................................... 63
15.4 Hot-air sterilization at 180 °C / 356 °F............................................................................................... 64
15.4.1 Overview ................................................................................................................................. 64
15.4.2 Procedure for hot-air sterilization: ........................................................................................... 64
15.5 Aborting the hot-air sterilization ........................................................................................................ 67
15.5.1 Aborting the hot-air sterilization manually ............................................................................... 68
15.5.2 Aborting the hot-air sterilization by opening the outer door .................................................... 69
15.5.3 Aborting the hot-air sterilization using the main power switch ................................................ 69
16. MAINTENANCE, AND SERVICE ......................................................................... 70
16.1 Maintenance intervals, service .......................................................................................................... 70
16.2 Checking the air jacket heating fan ................................................................................................... 70
16.3 Gas inlet fine filter ............................................................................................................................. 70
16.4 Sending the unit back to BINDER GmbH ......................................................................................... 71