C (E3) 08/2015 page 64/93
15.4 Hot-air sterilization at 180 °C / 356 °F
The very first sterilization after operation may cause an odor. This is not a quality defect. We
recommend ventilating well the room during sterilization.
15.4.1 Overview
Holding Cooling down
approx. 2
hours
4 hours approx. 6 hours
Sterilization cycle of approx. 12 hours
Figure 28: Set-point profile during the sterilization cycle
The C 170 can perform an automatically controlled hot-air sterilization cycle. This procedure will take
approx. 12 hours and consists of the following steps:
• Heating up phase: The incubator heats up the inner chamber as fast as possible to the sterilization
set-point temperature
• Holding phase: Constant sterilization set-point temperature
The sterilization set-point temperature is pre-set in factory to 190 °C / 374 °F. When this temperature
is reached, the holding phase begins. The duration of the holding phase is in total 4 hours. This en-
sures that 180 °C / 356 °F is maintained on all internal surfaces for at least 30 minutes.
• Cooling down phase: until 37 °C / 98.6°F is reached.
When 37 °C / 98.6 °F ± 1 ° is reached, the display "End" in the lower controller display (Figure 32 page
67) indicates the end of the sterilization procedure.
The precise duration of the entire sterilization cycle depends on the ambient temperature at the installa-
tion site and can thus vary. At an ambient temperature of 25 °C / 77 °F, the total duration is approx. 12
hours. You can shorten the cooling-down time by aborting the sterilization cycle (chap. 15.5) during the
cooling-down phase, i.e. no sooner than after 6 hours.
During sterilization, the CO
2
valve is closed and the CO
2
controller turns off entirely.
15.4.2 Procedure for hot-air sterilization:
Before carrying out the first hot-air sterilization, remove any protective lamination sheet from
the inner metal surfaces.
When starting a hot-air sterilization, CO
2
control automatically becomes inactive.
The safety controller settings are inactive during sterilization. They become functional again
following abortion of the sterilization and/or restart of the unit at the main power switch.
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