Operating Instructions RF-2000
24
4.7.2 Adjusting the light source position (fine adjustment)
Important:
If a pen recorder is connected, proceed as described below. If
not, please contact the Dionex Service for the fine adjustment of
the light source position.
(1) Connect a recorder to the 10mV RECORDER terminal of the RF-2000 (see
section 7.1), and turn the power ON.
(2) Remove the flow cell from the RF-2000. Pour distilled water into the flow cell
using the provided syringe. Make sure that no bubbles are present in the flow
cell, and install the flow cell in the sample compartment (see "8.1
Maintenance of Flow Cell").
(3) Set recorder parameter as follows:
1) Range : 10mV/Full Scale
2) Chart Speed : 10mm/min
(4) Adjust the zero position by pressing the zero key of the RF-2000.
(5) Adjust the zero position of the recorder.
(6) Press func key of the RF-2000 until the fluorescent character displays
LAMP ADJUST
(7) Press ENTER.
The parameters are automatically set as follows
(Refer to "5 Operation"):
1) EX : 350nm
2) EM : 450nm
3) GAIN : 2 ( x 4)
4) RANGE : 4 ( x 1/8)
5) RATIO : 0 (OFF)
6) sens : 2 (MED)
7) rsp : 1 (0.1s)
8) Z WAVE : 1 (OFF)
The above parameters are set and the auto-zero function is activated
automatically while the following message appears on the screen.
SETTING ZERO
Then the emission wavelength is changed and set at 397nm automatically
and the following message appears.
LAMP ADJUST XX.XX
Please note:
Be sure to obey the order of adjustment of the horizontal
direction adjusting screw and the vertical direction adjustment
screw as described below. If the order is not correct, the light
source position deviates greatly from the slit of the excitation
monochromator and re-adjustment cannot be possible. In this
case, carry out the adjustment from "4.7.1 Adjusting the light
source position (Coarse adjustment)" again.
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