For the TN system, carry out the following 8 service items and checks listed below:
Replace the tubing element (6.4mm OD, 3.2mm ID EMPP 562 tubes) of the
WMM60 Nitrogen Pump (N Pump). The WMM60 replacement tubes are
supplied in the service kits with fittings already attached (see item D in service
kit). The overall length of the tube and fittings should be 205.5mm, the tube
itself is 161.5mm.
Check that the peristaltic pumps are pumping correctly:
Nitrogen Pump (N Pump) rate for WMM60 Pump at 16 pulses should be
between 6.5ml and 7.5ml in ~8 seconds. The pump rate can be measured by
supplying DI Water or tap water to the pump at the inlet port using a small
container.
Check that the valves are operating correctly:
NP Sample Valve checked. LV3
DI Water Valve checked. LV2
TN Cleaning Valve checked. LV1
Check that the TN measuring cell is clean in the DCP module.
Check that the Xenon Lamp light source is functioning in the DCP module.
In Diagnostics, Process Test menu, run the READ DIW REF TEST. Confirm
that all Nitrogen Signal (N SIG) and Nitrogen Reference (N REF) % intensity
count values are greater than > 50%.
Open the CO
2
Analyzer metal box, clean CO
2
analyzer optics by removing the
4x M4x60 Allen bolts on the detector section. If necessary, using the Lens
Tissue (see item T in the service kits) clean both lenses which are located on
the detector and the source section of the CO
2
Analyzer. Replace o-ring with
the 72-0325-30 o-ring provided (see item P in the service kit).
Go to Oxidation Phase Simulate menu and set the MFC flow to 10 l/hr and flow
oxygen gas through the CO
2
analyzer for 5 minutes. At the end of this period,
the CO
2
analyzer zero reading (CO2) should be within ±0.5% of full scale of
the CO
2
analyzer range. For instance, if the CO
2
analyzer range is 10000ppm,
then the CO
2
analyzer zero reading should be typically ±50ppm.
If the CO2 reading is outside the 0.5%, check the purity of Oxygen gas. When
an oxygen concentrator is used, run the concentrator for at least 10 minutes
before the oxygen purity test is carried out. Confirm that there is no CO
2
in the
oxygen gas by connecting the CO
2
filter (used with the base container)
between the oxygen source and BioTector oxygen inlet and set the MFC to 10
l/h. As the size of the CO
2
filter is small, keep the 10 l/h gas flow running for at
least for 5 minutes and record the CO
2
zero readings at the end of the 5
minute period. If the CO
2
zero readings do not drop significantly with the CO
2
filter in place, this will indicate that there is no CO
2
contamination in the oxygen
supply.
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