1. Select the More Apps icon from the home screen and the
Absorbance/Ratio icon from the More Apps screen
2. To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3. For NanoVolume application:
Select the dilution depending on the sample concentration
Note: There is no automatic path length setting in this method. Select either a virtual
dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).
For cuvette application:
Select the path length depending on the used cuvette.
Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm
If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater.
When the cuvette holder has reached 37°C the toggle color changes to green.
Note: Only available for cuvette applications (NP80 and C40).
For NanoVolume application with submicroliter cell (C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different
lids see page 52 Submicroliter Cell Measurements Basics). Required sample volume for
each lid is shown.
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