NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
114
MORE APPS: CONCENTRATION
METHOD OVERVIEW
In this mode, concentration can be calculated for a sample by determining the absorbance at
a specific wavelength relative to a reference. The concentration is then obtained by
multiplying the measured absorbance by a specific factor. This factor may be known in
advance and entered by the user, or it may be calculated by the instrument by measuring a
set of standard (standard curve method) with known concentrations to create a standard
curve.
MEASUREMENT PROTOCOL
1. Select the More Apps icon from the home screen and the
Concentration icon from the More Apps screen.
2. To change between NanoVolume and Cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3. For NanoVolume application:
Select the dilution depending on the sample concentration.
Note: There is no automatic path length setting in this method. Select either a virtual
dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).
For cuvette application:
Select the path length depending on the used cuvette.
Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm
If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder
heater. When the cuvette holder has reached 37°C the toggle color changes to green.
Note: Only available for cuvette applications (NP80 and C40).
For NanoVolume application with submicroliter cell (C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different
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