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NanoPhotometer

N120/NP80/N60/N50/C40 User Manual Version 4.3.1

BUTTONS

When opening a method and starting a sample measurement, a blank

measurement is required. For the blank measurement, either water or

sample buffer can be used to give the NanoPhotometer

a reference of

what zero should be. It is recommended to re-apply the blank solution

and measure it as a sample to ensure the graph of the blank spectra is

a flat line.

To initiate the spectral scan of the sample push the sample button. The

data will be temporarily stored until the method is exited; at this time the

user needs to define if the samples should be saved.

The auto sample button is disabled by default. When switched on

sample measurements are started automatically as soon as the lid arm

is closed. Auto sample function is only available for sample and not for

blank measurements.

Note: Auto sample function is only available NanoVolume methods and

new NanoPhotometer

versions it may not be available after updating

an older firmware version.

The Change to Cuvette button (NP80) is located at the bottom of the

parameter window. The change to Cuvette/NanoVolume button toggles

between NanoVolume and cuvette applications. Selecting the Change

to Cuvette button activates the cuvette compartment (opens the sliding

cover and switches the red arrow LED on). Only cuvette measurements

are possible when this mode is selected. Selecting the Change to

NanoVolume button while the cuvette port is activated will deactivate

the cuvette compartment (closes the sliding cover and switches the LED

off) and only NanoVolume measurements are possible.

Note: Please make sure that the lid arm is closed during cuvette measurements.

The Change to NanoVolume button (C40) is only shown when

submicroliter cell applications are enabled in the preferences. The

Change to NanoVolume/Cuvette button toggles between cuvette and

submicroliter cell applications. With the NanoVolume submicroliter cell

application the parameter of each method shows a list for lid selection

(Lid 5, 10, 50, 100 and 250). Choose the correct lid and the results are

automatically calculated considering the virtual dilution.

Implen NanoPhotometer N120 User Manual

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