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NanoPhotometer

N120/NP80/N60/N50/C40 User Manual Version 4.3.1

118

MORE APPS: STANDARD CURVE

METHOD OVERVIEW

The construction of a calibration curve from multiple standards of known concentrations can

be created and stored on the NanoPhotometer

. The standard curve can be used to quantify

samples of the same type with unknown concentrations. This application provides an

extremely useful tool with which to integrate, expedite and simplify the measurement and

calculations involved in determining the concentration of analytes in unknown samples. If a

zero concentration standard is required, include it in the number of standards to be entered

using a reagent blank and entering 0.00 for concentration.

MEASUREMENT PROTOCOL

1. Select the More Apps icon from the home screen and the Standard

Curve icon from the More Apps screen.

2. To change between NanoVolume and Cuvette application, use the

Change to Cuvette/NanoVolume button below the parameter area

(NP80/C40 only).

3. For NanoVolume application:

Select the dilution depending on the sample concentration

Note: There is no automatic path length setting in this method. Select either a virtual

dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).

For cuvette application:

Select the path length depending on the used cuvette.

If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater.

When the cuvette holder has reached 37°C the toggle color changes to green.

Note: Only available for cuvette applications (NP80 and C40).

For NanoVolume application with submicroliter cell (C40 only):

Implen NanoPhotometer N120 User Manual

Questions and Answers: