NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
100
8. Option to set/calculate a dilution factor for manual diluted samples.
9. Insert cuvette with the reference sample and select blank button to initiate
the measurement
10. Insert cuvette with the sample and select the sample button to initiate the
measurement.
CALCULATIONS
OD600 = A
600
* Ð * cf
OD600 Optical density at 600 nm
A
600
Absorbance at 600 nm (10 mm path)
Ð Dilution factor
cf
Correction factor for spectrophotometer
Cells ml
= A
600
* Ð * cf * multiplier
A
600
Absorbance at 600 nm (10 mm path)
Ð Dilution factor
cf
Correction factor for spectrophotometer
multiplier Multiplier of sample