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NanoPhotometer

N120/NP80/N60/N50/C40 User Manual Version 4.3.1

4. NANOPHOTOMETER

APPLICATIONS

The NanoPhotometer

comes with pre-programmed applications as well as the ability to

create custom applications. Any application method can be selected by tapping the icon

once or pushing on the icon (computer based software).

Note: Within each method selection of NanoVolume or cuvette mode (NP80/C40) is

possible. The “Change to Cuvette” or “Change to NanoVolume” button is located at the

bottom of the parameter area.

NUCLEIC ACIDS

METHOD OVERVIEW

Nucleic acids in solution absorb light with a peak in the ultraviolet region of 260 nm. For

determination of nucleic acid concentration in solution the absorbance at wavelength 260 nm

is used along with the Beer-Lambert law. In addition to calculating concentrations of nucleic

acids, absorbance measurements are also useful for estimating purity of nucleic acids by

calculating the 260/280 nm and 260/230 nm ratios. Further, it is possible to determine the

degree of labeling of nucleic acids with probes including fluorescent dyes.

Sample Control™ gives useful information about sample conditions. It recognizes air

bubbles, sample impurities, turbidity, lint residues and potential contaminations. If Sample

Control™ detects any inconstancy an alert icon is shown in the result/table area. A push

on the alert icon shows additional information about the inconstancy.

MEASUREMENT PROTOCOL SINGLE SAMPLE

1. Select the Nucleic Acids icon on the home screen.

2. To change between NanoVolume and cuvette application (NP80/C40

only) use the Change to Cuvette/NanoVolume button below the

parameter area.

3. To change the nucleic acid type push on dsDNA and a list with available options opens on

the right side.

Options are: dsDNA, ssDNA, RNA miRNA, miRNA Sequence, Oligo, Oligo Sequence and

Custom (see Table 1 on page 66).

• The miRNA sequence and Oligo sequence options allow the sequence to be entered

and the extinction coefficient will be automatically calculated. A minimum of 4 bases is

required.

• The default setting for Custom is 50 and allows changing the extinction coefficient (1/Ɛ)

in a range of 15 - 150. It is possible to enter a name for the custom nucleic acid factor

for documentation.

Implen NanoPhotometer N120 User Manual

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