NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
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3. NANOPHOTOMETER
®
BASICS
The NanoPhotometer
®
product line offers a complete solution for NanoVolume
(N120/NP80/N60/N50/C40) and standard cuvette (C40/NP80) applications. The
NanoPhotometer
®
N120 is a multi-sample model that can measure up to 12 samples per
run. The multi-channel option is available in Nucleic Acid, Protein UV, Protein Assay and
Wavescan method.
NanoVolume applications start with a minimum sample volume of 0.3 μl (NP80/N60/N50)
and 2 µl for the NanoPhotometer
®
N120. Standard cuvette applications can be performed
with 10 mm, 5 mm, 2 mm, 1 mm and 0.5 mm path length quartz, glass, or plastic cuvettes
with a center height of 8.5 mm.
APPLICATIONS OVERVIEW
The NanoPhotometer
®
comes with pre-programmed applications as well as the ability to
create custom applications. To select a method, tap the corresponding icon and the method
opens immediately.
Concentration, purity, and dye incorporation for DNA, RNA,
Oligo, and other nucleic acids
Protein UV determination at 280 nm (or in a range of 200 -
330 nm), purity and dye incorporation
Time vs. Absorbance readings