NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
117
11. Use a lint-free laboratory wipe to clean both the sample window on
pedestal and mirror in lid arm prior to applying the next sample.
Note: It can be helpful to apply the blank a second time and read it as a
sample to ensure a proper blank.
12. Apply sample to the sample window on pedestal and press the sample
button to initiate the measurement.
If auto sample is active the sample measurement is automatically started
after closing the lid arm.
Note: Auto sample function is only available NanoVolume methods and
new NanoPhotometer
®
versions it may not be available after updating an
older firmware version.
CALCULATIONS
In this method, the concentration of the sample is calculated based on the Beer-Lambert law
given the user specified wavelength of interest and user defined extinction coefficient. The
equations for calculating concentration without background correction are as follows:
Without background correction:
C Concentration (ng/µl)
Absorbance at user specified path length n (10 mm path)
Dilution factor
ε extinction coefficient/factor
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