NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
68
With background correction without dye correction:
FOI =
324.5 *(A
max, dye
A
)
ε
dye
* 10
6
* (A
260
A
BKG
) * ε
nuc
Without background correction and with dye correction:
FOI =
324.5 *A
max, dye
ε
dye
* 10
6
*(A
260
cf
dye
* A
max,dye
) *ε
nuc
Without background correction and without dye correction:
FOI =
324.5 * A
max, dye
ε
dye
* 10
6
* A
260
* ε
nuc
FOI Frequency of Incorporation (dye per 1,000 bases)
A
260
Absorbance at 260 nm (10 mm path)
A
BKG
Absorbance at selected background wavelength (10 mm path)
A
max,dye
Absorbance value at the absorbance maximum of the dye (10 mm path)
ε
dye
Extinction coefficient of dye in M
-1
*cm-
1
ε
nuc
Extinction coefficient/nucleic acid factor in ng*cm/µl
cf
dye
Dye-dependent correction factor at 260 nm
Table 2. Dye Types, Absorbance Max, Extinction coefficient, and dye-dependent
correction factors
Absorbance
maximum of
Dye (nm)
Dye-dependent
ext. coeff.
ε
dye
in M
-1
*cm
-1
Dye-dependent
correction factor
at 260 nm cf
dye
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