NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
71
MEASUREMENT PROTOCOL SINGLE SAMPLE
1. Select the Protein UV icon on the home screen.
2. To change between NanoVolume and Cuvette application (NP80/C40
only) use the Change to Cuvette/NanoVolume button below the
parameter area.
3. To change the protein type push on BSA and a list with available options opens on the right
side.
Options are: BSA, SA Mouse, SA Human, IgG Mouse, IgG Human, IgE Human, Lysozyme,
OD1, Custom (Molar Extinction Coefficient), Custom (Extinction Coefficient) and Custom
(1/Ɛ).
For Custom (Mol. Ext. Coefficient) enter Molecular Weight in g/mol and the Mol. Ext.
Coefficient in M
-1
*cm
-1
For Custom (Ext. Coefficient) enter the Ext. Coefficient in l/g*cm
For Custom (1/Ɛ) enter the calculated protein factor 1/Ɛ
4. For NanoVolume application:
Select the volume of sample to be applied.
Note: 1-2 µl (default): automatic path length setting; 0.3 µl measures only the 0.07 mm
path length (possible for samples with concentrations e.g. BSA > 12.6 mg/ml)
For cuvette application:
Select the path length depending on the used cuvette.
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