NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
86
For NanoVolume application with submicroliter cell (C40 only):
Select Lid depending on your sample concentration (for concentration ranges of different
lids see page 52 Submicroliter Cell Measurements Basics).
Required sample volume for each lid is shown.
Note: A new Blank is recommended when changing the dilution lid.
5. Default values for the baseline correction are depending on selected protein assay type and
instrument version:
BCA default value 750 nm (N50 off)
Biuret default off
Bradford default value 750 nm (N50: 350 nm)
Lowry default value 405 nm
Note: It is recommended to use the default baseline for each assay.
6. Select curve fit type: Options are linear regression, zero regression (forces the straight line
through the origin) and 2
nd
order regression
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