NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
75
Options are: BSA, SA Mouse, SA Human, IgG Mouse, IgG Human, IgE Human,
Lysozyme, OD1, Custom (Molar Extinction Coefficient), Custom (Extinction Coefficient)
and Custom (1/Ɛ).
For Custom (Mol. Ext. Coefficient) enter Molecular Weight in g/mol and the Mol. Ext.
Coefficient in M-1*cm-1
For Custom (Ext. Coefficient) enter the Ext. Coefficient in l/g*cm
For Custom (1/Ɛ) enter the calculated protein factor 1/Ɛ
4. The wavelength for protein measurements can be changed in the range
of 200 – 330nm depending on the wavelength peak of the protein. Default
setting is 280 nm.
5. Select the Units in which the concentration should be calculated. Options are ng/µl, µg/µl,
µg/ml and mg/ml (default).
6. The background correction is enabled at 320 nm by default. It is possible to disable the
background correction with the toggle switch. Selection options are 320 nm, 340 nm or
any wavelength in the range of 220 – 350 nm. The background correction can be disabled
with the toggle switch.
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