ASSIST PLUS – Operating instructions V02 Programming
38 INTEGRA Biosciences AG
When finished, press to save the Custom program. To run the program, press OK.
Step Description of Custom Program
Move X Moves the pipette by the defined distance in X-direction relative to the
current position by X mm. Setting a negative value (mm) moves it to the
left, setting a positive value (mm) moves it to the right.
The distance from well to well for a 96 well plate is 9 mm and for a 384 well
plate 4.5 mm.
Move Z Moves the pipette in Z direction to the selected coordinate (the higher the
value, the longer is the distance between pipette tip and deck). Use the
Arrow keys on ASSIST PLUS to teach the position or dial the height
manually on the pipette’s screen. Press OK on the pipette to accept the
settings.
Move Y Moves the pipette in Y-direction to accommodate even and odd rows/
columns of plates using a pipette that does not correspond directly to the
row/column number. Setting a positive value (mm) moves it to the rear,
setting a negative value (mm) moves it to the front.
BlowOut Performs a blow out. A blow out needs to be performed after the last
dispense to remove liquid that may cling to the tips.
Note: When using “Purge” to empty the tips, a blowout/blowin is performed
automatically and does not need to be programmed.
BlowIn After a blow out, a blow in has to follow at some point. It does not have to
follow immediately and can have steps in between. E.g. after the blow out
a move step can be programmed to move the tips out of the liquid, and is
then followed by the blow in.
Delay A delay is a pause between one step and the next step. Define a delay
time (in seconds) or select Press RUN, i. e. pressing the RUN key is
needed to continue.
Loop A loop repeats the steps between the selected step and the loop command
as many times as defined. E.g. if the program reaches the loop step, it
goes back to step 3 and repeats the steps until there 2 times.
The number of program steps can often be shortened by adding a loop.
Loops inside loops are not allowed.
Call Calls another custom program to run as subprogram in the current program.
This can be utilized to run frequently used sequences, e.g. a tip touch after
a dispense, without programming the individual steps every time.
TipLoad Moves the pipette to the tip rack and attachs new GripTips. The pipette
moves automatically to the required position.
TipEject Ejects the GripTips into the waste bin. The pipette moves automatically to
the required position.
Beep Sets a beep. The sound is only active, if under Preferences - Sounds the
option Messages is set to On.
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