ASSIST PLUS – Operating instructions V02 Programming
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Example of custom program
Application: The task is to aspirate liquid from a 100 ml reservoir with a 300 µl pipette
and fill the first 6 columns of a 96 well plate with 50 µl. The custom program would be set
up as follows:
1) 2), 3) 4) 5)-8) 9)
Program step Action
1) TipLoad Tips are automatically loaded and the pipette moves
up.
2) Move X,Z: 180.2; 20.0 Move to absolute X,Z-position to aspirate liquid from the
reservoir (to the right and down).
3) Aspirate: 300 µl,
Tip Travel: 3.0 mm,
Speed: 8
Aspirate liquid from 100 ml reservoir.
4) Move X,Z: 242.5; 50 Move to absolute X,Z-position above first row (up =
clearance height reservoir, and to the right).
5) Move Z 10.0 mm Move down to absolute Z-position 10.0 mm for
dispense.
6) Dispense: 50 µl,
Tip Travel: 3.0 mm,
Speed: 8
Dispense liquid with Tip Travel 3 mm.
7) Move X: 5.0 mm Move 5 mm beside for Tip touch on the side of the well
(height at coordinate 13.0 mm due to Tip Travel).
8) Move X: -5.0 mm Move back to the middle of the well.
9) Move Z: 16.0 mm Move up to coordinate 16 mm (clearance height).
10) Move X: 9.0 mm Move 9 mm beside (center of next wells).
11) Loop: 5
Number of loops: 6
Repeat steps 5-10 (five times) to dispense a total of 6
columns of a 96-well plate.
12) TipEject Eject tips.
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