3. Getting Started
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3.8.4 Indenting and Releasing Program Elements
The decision to indent/release a program element will modify the workflow of the
instrument during measurements.
The actions of all program elements with the same indentation are performed
sequentially. The only dependence between these program elements is that the
next action starts directly after the previous action is finished.
A program element that is indented more than the previous program element
shows dependence between the two program elements. This means the
parameters defined in the first program element are also active for the second
(indented) program element.
The following is an example of how to define a Multilabel kinetic with two
Absorbance labels. The example shows that the two Absorbance program
elements depend on the Kinetic Cycle program element, which depends on the
Part of Plate program element, which depends on the Plate program element.
Define the parameters for an example as follows:
1. Plate: e.g. Greiner 96 Flat Transparent
2. Kinetic Cycle/Number of cycles: 5
3. Absorbance/Wavelength Label 1: 450 nm
4. Absorbance/Wavelength Label 2: 492 nm
The Workflow pane appears as shown in the screenshot:
The definition above results in the following workflow:
The absorbance of all wells of the 96-well microplate is first measured at 450 nm
and then at 492 nm. Both absorbance measurements are performed in 5 kinetic
cycles.
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