6. Application Example
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6.2.6 Summary of Definition of Quantitative ELISA in LT-com
1. Subtract Blank value
Definitions in LT-com
Click on Add new transformation in the control bar and a window appears,
asking if you want to define a Blank reduction. Click Yes and the Blank
reduction formula is assigned automatically to all wells.
2. Define Concentrations
Definitions in LT-com (Control bar – Method layout/ Conc.-,
Dil.-, Ref.-values)
Selected identifier: ST
Unit: UA/ml
ST1_1 5 (ST1_1 ..Standard 1 first experimental group)
ST1_2 10 (ST1_2 ..Standard 2 first experimental group)
ST1_3 20 (ST1_3 ..Standard 3 first experimental group)
ST1_4 40 (ST1_4 ..Standard 4 first experimental group)
ST1_5 80 (ST1_5 ..Standard 5 first experimental group)
ST1_6 160 (ST1_6 ..Standard 6 first experimental group)
3. Define Standard Curve
Definitions in LT-com (Control bar – Concentrations/ Standard
curve)
Input data blank reduction
Analysis type linear regression
X-axis linear
Y-axis linear
4. Define Cutoffs
Definitions in LT-com (Control bar – Evaluate data/ Cutoff
definition)
Input data: Mean conc. (UA/ml)
Limits: 22
18
Positive ≥ 22 > intermediate ≥ 18 > negative
Non-competitive test
5. QC Validation
Definitions in LT-com (Control bar – Evaluate data/QC
validation):
Input data: Single conc. (UA/ml)
Validation condition 1 NC1_1<8
Validation condition 2 NC1_2<8
NC1_1 ..Negative Control first replicate first experimental group
NC1_2 ..Negative Control second replicate first experimental group
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