6. Application Example
50
Measurement and Evaluation
Read plate at a wavelength of 492 nm, reference at 620 nm.
Blank reader/plate on well A1.
Concentrations of the Calibrators (Standards):
Calibrator 1 5 UA/ml
Calibrator 2 10 UA/ml
Calibrator 3 20 UA/ml
Calibrator 4 40 UA/ml
Calibrator 5 80 UA/ml
Calibrator 6 160 UA/ml
After the blank correction the optical densities (OD 492 - OD 620) are plotted
versus the concentration. The regression line that goes through these points is
the standard curve.
Interpretation of the test results:
IgM < 18 UA/ml Negative
18 UA/ml ≤ IgM < 22 UA/ml Intermediate
IgM ≥ 22 UA/ml Positive
The calculated IgM concentration of both negative controls must be under
8 UA/ml.
Data Handling
After the measurement, the data file (workspace) is stored automatically and a
report containing the measurement parameters, plate layout, blanked values,
standard curve, IgM-concentrations, cutoff definition, qualitative results of the
samples and validations is created.
Additionally, the layout and the qualitative results are being stored as ASCII file.
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