Metrohm 761 Compact IC - External Standard Calibration; Component Table

4.4 Methods
761 Compact IC
119
External standard calibration
External standard calibration (absolute calibration) is a key way of cali-
bration. Basically this procedure calculates the dependence of injected
component's quantity versus area (or height) of the corresponding
peak. This dependence is showed as a calibration plot with injected
quantity along the Y axis and peak area (or height) along the X axis. The
injected quantity Q
i
is calculated as product of component's concentra-
tion C
i
to the reduced injected sample volume V':
Q
i
= C
i
• V'
The calibration function W
i
(R) for each component is calculated using
the RSD method.
W
i
(R) = RSD(Q
ij
, R
ij
) = RSD((C
i
• V'), R
ij
)
When quantification procedure is performed, a concentration C
i
(abso-
lute concentration of a component in the sample) is determined as a ra-
tio of the computed quantity W
i
(R
i
) to the corrected volume of sample
injected V':
C
i
= W
i
(R
i
) / V'
Component table
761 COMPACT IC / Method / Calibration / Components
If the component table is opened, the chromatogram window is split
into two parts. The upper one shows the chromatogram, the compo-
nent table appears in the bottom part of the screen. When moving into
the component table, a special cursor in the upper part of the window
jumps to the peak corresponding to the current component.
The
Components table stores the following information on the compo-
nents to be analyzed: