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General| Plate Formats | 6- to 384-well microplates |
|---|---|
| Software | SoftMax Pro Software |
| Type | Multi-mode microplate reader |
| Detection Modes | Absorbance, Fluorescence, Luminescence |
| Pipettor | 8-channel pipettor |
| Wavelength Selection | Monochromators |
| Temperature Control | 4 °C above ambient to 45 °C |
Background on the system, its components, and functional overviews.
Guidance on effectively using the user manual for safe and proper system operation.
Details potential hazards and essential safety guidelines for users.
Provides a comprehensive overview of the FlexStation 3 instrument's capabilities and functions.
Describes the major hardware parts, including panels, drawers, and modules.
Outlines the fundamental steps for operating the FlexStation 3 instrument.
Explains the underlying principles of the instrument's design and how it functions.
Introduces the integrated software used for controlling the instrument and analyzing data.
Essential safety warnings and precautions before starting any installation procedures.
Step-by-step instructions for safely unpacking the instrument and its components.
Detailed guide on how to correctly install the fluidics module.
Procedures for installing the 8-channel or 16-channel pipettor head.
Guidance on setting up the host computer and monitor for the instrument.
Instructions for connecting necessary cables between the instrument and computer.
Steps for installing microplate and compound baseplate adapters.
Instructions for installing the required SoftMax Pro software.
Lists the basic operating procedures for using the FlexStation 3 instrument.
Steps for powering on the instrument and initiating system startup.
Explains how to interact with the instrument using its front panel controls.
Details how to set and manage the reading chamber temperature.
Guides users through configuring instrument status and settings in the software.
Covers essential parameters for optimizing assay data acquisition.
Specific parameters for configuring fluid transfer operations with the integrated pipettor.
Procedures for loading and initiating microplate reads.
Instructions on how to begin a microplate reading experiment.
Guidance on adjusting settings for optimal fluorescence assay performance.
Tips for optimizing TRF assay results.
Strategies for optimizing FP assay performance.
Recommendations for optimizing luminescence assay measurements.
Information on how to contact Molecular Devices for technical assistance.
Steps for safely relocating the instrument.
Procedures for cleaning the exterior surfaces and internal components.
Instructions for handling fluid spills within the instrument.
Guide on how to clean the instrument's fan filter.
Routine cleaning procedures for the pipettor head barrels.
Instructions for installing and removing microplate adapters.
How to install and use the compound baseplate.
Steps for replacing blown fuses in the instrument.
Procedures for replacing the instrument's flash lamp.
Guidance for resolving issues encountered during system startup.
Instructions for manually opening instrument drawers in specific situations.
Explains common mechanical issues and how to avoid them.
Steps to recover from mechanical failures during fluidics operations.
How to identify and diagnose mechanical issues based on error messages.
Procedures for examining the fluidics module for problems.
How to remove residual fluid from pipette tips.
Steps for restoring communication after an instrument malfunction.
Information on interpreting and responding to instrument error codes.
Details on handling additional error messages not covered elsewhere.
Procedures for tilting or removing the fluidics module for cleaning or tip recovery.
Lists part numbers and descriptions for system components and accessories.
Technical details for the 8-channel pipettor's performance.
Technical details for the 16-channel pipettor's performance.
Performance specifications related to absorbance measurements.
Performance specifications for fluorescence intensity detection.
Performance specifications for fluorescence polarization measurements.
Performance specifications for time-resolved fluorescence assays.
Performance specifications for luminescence measurements.
Specifications regarding the instrument's temperature control capabilities.
Defines absorbance as the amount of light absorbed by a solution.
Explains the Automix function for automated microplate shaking.
Describes an emission cutoff filter's role in conditioning light.
Defines an emission spectrum scan for measuring fluorescence/luminescence.
Defines an Endpoint read as a single measurement per well.
Defines an excitation filter's function in reducing light.
Defines an excitation spectrum scan for measuring fluorescence.
Defines a fluorophore as a light-absorbing, light-emitting molecule.
Describes the incubator function for temperature regulation.
Defines instrument setup as defining parameters for reading microplates.
Defines a Kinetic read as collecting data over time at intervals.
Defines luminescence as light emission from non-thermal processes.
Defines a PMT as a light detector.
Explains read mode as the type of measurement performed.
Defines read type as the method for reading a microplate.
Describes the integrated software for instrument control and data collection.
Defines trituration as mixing well contents by aspiration/dispensing.
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