Molecular Devices FlexStation 3 - Fluorescence Intensity Performance; Fluorescence Polarization Performance

Performance Specifications
0112-0127 B 157
Table B-4: Fluorescence Intensity Performance
Item Specification
Sensitivity Top Read
< 5 pM FITC, 1 fmol/200 μL (96)
< 20 pM, 2 fmol/100 μL (384)
Wavelength range 250 nm to 850 nm
Wavelength selection Monochromators, tunable in 1 nm increments
Bandwidth (excitation,
emission)
9 nm, 15 nm
Number of
excitation/emission pairs
per plate
4
Dynamic range 106 in 96-well black plates: auto gain circuitry
System validation Self-calibrating with built-in fluorescence calibrators
Light source Xenon flash lamp (1 joule/flash)
Average lamp lifetime 2 years normal operation
Detector Photomultiplier
Table B-5: Fluorescence Polarization Performance
Item Specification
Wavelength range 400 nm to 750 nm
Wavelength selection Monochromators, tunable in 1 nm increments
Bandwidth (excitation,
emission)
9 nm, 15 nm
Precision < 5 mP standard deviation at 1 nM fluorescein
in 96-well and 384-well microplates
FlexStation_manual.book Page 157 Tuesday, June 25, 2013 10:22 AM