Operating Procedures
0112-0127 B 79
Read Types
Choose from five read types. The reading can occur in a each well of a
microplate unless otherwise noted.
• Flex: Data is collected over a specified period of time at regular
intervals and uses integrated fluid transfer to delivery reagents
while the wells are being read.
• Endpoint: A single reading is taken. Fluid transfer can occur only
before the read.
• Kinetic: Data is collected over a specified period of time at
regular intervals and fluid transfer can occur only before the first
read.
• Spectrum: The scanning monochromator is used to take readings
of the same sample at many different wavelengths within the
range specified. No fluid integrated transfer is available.
• Well Scan: Multiple readings are taken in different areas of each
well in a 6-well to 96-well microplate. No integrated fluid transfer
is available.
Read Modes
The detection modes available for each experiment will vary depending
on the read type selected. Select from up to five possible Read Modes:
• Fluorescence (RFUs)
• Absorbance (ODs)
• Luminescence (RLUs)
• Time-Resolved Fluorescence (RFUs)
• Fluorescence Polarization (RFUs)
If bottom reading is possible for the selected read mode, a check box
appears in the window that says Well Bottom Read. If you want to read
through the bottom of a clear-bottom plate, select the Well Bottom
Read check box.
FlexStation_manual.book Page 79 Tuesday, June 25, 2013 10:22 AM
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