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FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
16
0112-0127 B
Figure 1-3:
Instrument, Fr
ont View
Figure 1-4:
Instrument, R
ear View
FlexStation_manual.book Page 16 Tuesday, June 25, 2013 10:22 AM
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17
Table of Contents
Main Page
Default Chapter
3
Table of Contents
3
Chapter 1: Description
7
Introduction
7
How to Use this User Guide
9
User Guide Organization
9
Safety Information
9
Conventions Used for Precautionary Information
10
Electrical Safety
10
Service-Trained Users
11
Avoiding Mechanical Problems During Fluid Transfer
11
Safety Messages
11
System Overview
13
System Components
15
Covers and Instrument Panels
17
Drawers
21
Fluidics Module
24
Detection Module
26
Computer
28
Accessories
28
Consumables
30
Overview of Operation
32
Choosing an Experiment
32
Preparing the Instrument
32
Preparing the Software
33
Running the Experiment
33
Analyzing the Data
33
Theory of Operation
33
Instrument Design
33
Assay Read Types
38
Softmax Pro Software
41
Set up the Reader and Software Parameters
42
Acquire Data from the Reader
43
Perform Complex Data Analysis
44
User Interface
45
Flexstation 3 Benchtop Multi-Mode Microplate Reader User Guide
48
Chapter 2: Installation
49
General Precautionary Information
49
Unpacking the System
50
Unpacking the Fluidics Module and Accessories
51
Unpacking the Detection Module
51
Removing the Shipping Screws
52
Installing the Fluidics Module
53
Installing the Pipettor Head
56
Setting up the Computer
61
Connecting the Cables
61
Installing the Drawer Adapters
62
Microplate Adapter Installation
62
Compound Baseplate Installation
63
Installing Softmax Pro
63
Chapter 3: Operating Procedures
65
Overview
66
Starting up the System
67
Using the Control Panel
70
Setting the Temperature
72
Displaying the Temperature
72
Setting the Temperature with the Control Panel
73
Setting the Temperature with Softmax Pro Software
74
Setting up the Software
75
Softmax Pro Software Parameters
78
Read Types
79
Read Modes
79
Wavelengths
80
Sensitivity
80
Timing
81
Assay Plate Type
82
Wells to Read
83
Automix
84
Autocalibrate
85
Settling Time
85
Autoread
85
Softmax Pro Software Parameters for Fluid Transfer
86
Compound Source
87
Compound Transfer
88
Triturate Selection
92
Pipette Tips Layout
93
Compound and Tip Columns
93
Pipette Tip Air Gap
97
Settings Displayed in Plate Sections
98
Other Software Settings
99
Reading a Microplate
99
Loading Tips and Microplates
99
Starting the Reading
100
Selecting a Plate
101
Replacing Data in a Plate
101
Viewing Experiment Progress
102
Data Display
102
Shutting down the System
103
Optimizing Fluorescence Assays
103
Using Spectral Scanning to Optimize Excitation and Emission Wavelengths for Fluorescence Assays
105
Optimizing Time-Resolved Fluorescence Assays
110
Optimizing Fluorescence Polarization Assays
111
Optimizing Luminescence Assays
112
Chapter 4: Maintenance
113
Obtaining Support
114
Moving the Instrument
114
Cleaning the Instrument
115
Cleaning up Spills
116
Cleaning the Fan Filter
116
Cleaning the Barrels on the Pipettor Head
117
Using the Microplate Adapters
120
Microplate Adapter Installation
120
Removing the Microplate Adapter
120
Using the Compound Baseplate
121
Replacing Fuses
121
Replacing the Flash Lamp
123
Long-Term Shutdown
128
Chapter 5: Troubleshooting Procedures
129
Problems During Startup
130
Opening a Drawer Manually
132
Understanding Potential Mechanical Problems
133
Background
133
Before Using the Instrument
133
Avoiding Mechanical Problems
134
In Case of Power Failure
135
Fluidics
135
Recovering from Mechanical Problems in Flex Mode When Using Fluidics
135
Assessing a Mechanical Problem
136
Opening the Instrument
137
Evaluating the Tip Rack
138
Inspecting Inside the Fluidics Module
139
Removing the Pipettor Head
142
Expelling Undispensed Fluid from Tips
145
Recovery Procedure
146
General Error Messages
147
Error Messages
147
Other Error Messages
148
Tilting or Removing the Fluidics Module
149
Tilting the Fluidics Module
149
Removing the Fluidics Module
151
Appendix A: Parts and Accessories
153
Ordering Parts and Accessories
154
Appendix B: Performance Specifications
155
Glossary
163
Index
167
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