Nikon A1 User Manual

2
CONTENTS
Chapter 1. Introduction ...............................................................................................................................................3
Basic Operation ............................................................................................................................................... 4
Chapter 2. Startup....................................................................................................................................................... 5
Chapter 3. Operation of the Microscope.....................................................................................................................7
Chapter 4. Capturing Color Images (Standard Detector)..........................................................................................13
Chapter 5. Capturing Multistained Images: Cross Talk Reduction (Standard Detector)............................................18
Chapter 6. Capturing Confocal Zoom ....................................................................................................................... 22
Chapter 7. Capturing ROI Scan and CROP Scan.....................................................................................................24
Chapter 8. Capturing Z Series Images .....................................................................................................................27
Chapter 9. Capturing Z Series Images (While Changing Brightness).......................................................................30
Chapter 10. Creating Three-Dimensional Image........................................................................................................32
Chapter 11. Creating a Slice View Image and a Projection Image .............................................................................35
Chapter 12. Capturing Time Series Images................................................................................................................36
Chapter 13. Time Measurement.................................................................................................................................38
Chapter 14. Capturing Photo Activation Imaging (Galvano Scanner / Time-Series Activation)................................... 40
Chapter 15. Image Display Function ..........................................................................................................................43
Chapter 16. Extracting ND2 Files...............................................................................................................................47
Chapter 17. Exporting ND2 Files................................................................................................................................48
Chapter 18. Shutdown................................................................................................................................................ 50
Spectrum Imaging ......................................................................................................................................... 51
Chapter 19. Capturing Spectral Images (Spectral Detector)....................................................................................... 52
Chapter 20. Separating Spectral Image (Unmixing) ...................................................................................................57
Chapter 21. Live Unmixing (Spectral Unmixing for Live Image) ................................................................................. 61
Chapter 22. Capturing Virtual Filter Images (Spectral Detector).................................................................................63
Motorized Stage ............................................................................................................................................. 69
Chapter 23. Capturing Multipoint Time Series Images ...............................................................................................70
Chapter 24. Capturing Large Images .........................................................................................................................72
High-Speed Imaging...................................................................................................................................... 77
Chapter 25. Capturing High-Speed Images (Resonant Scanner)............................................................................... 78
Chapter 26. Capturing High-Speed ZT Series Images (Resonant Scanner) ..............................................................82
Chapter 27. Capturing Simultaneous Photo Activation Imaging (Resonant & Galvano Scanner)...............................86

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available