Nikon A1 - 23 Capturing Multipoint Time Series Images; 23.2 Set image acquisition points.

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Capturing Multipoint Time Series Images
23
Chapter 23. Capturing Multipoint Time Series Images
23.1 Perform Steps 4.1 to 4.7 in Chapter 4, “Capturing Color Images” to determine image
acquisition conditions.
23.2 Set image acquisition points.
(1) Click the
[XYZ Time...] button to open the ND Acquisition dialog box.
(2) Select the [XY] tab.
(3) Move the point to the image acquisition point by using the joystick on the motorized stage while checking
the live image.
(4) Check the checkboxes in the [Point Name] column and register position information.
Checking the [Include Z] checkbox also registers the Z position.
The Z position of the microscope or the offset value (tracking focus position) of the perfect focus system
can be registered.
Note: If the checkboxes in the [Point Name] column are not displayed, click
.
(5) Repeat Steps (3) to (4) to register points as needed.
Coarse
Fine
+Y
+X-X
-Y
Ex Fine
Coarse
Fine
Ex Fine
Z Speed
XY Speed
Constant Speed
(6) Check the [Time] tab.
(7) Determine [Interval] (time interval) and
[Duration] (duration time).
(8) Check the [Save to File] checkbox to acquire
images while saving them.
Note: Images are saved in nd2 file format.
Note: We recommend acquiring time series
images while saving them.
(8)
(6)
(7)
(3), (5)-2 Joystick Controller
(1)
(2)
(4)-1
(5)-1
(
4
)
-2
(4) Note