Nikon A1 User Manual

27
Capturing Z Series Images
8
Chapter 8. Capturing Z Series Images
8.1 Perform Steps 4.1 to 4.7 in Chapter 4, “Capturing Color Images” to determine image
acquisition conditions.
Note: We recommend that image acquiring conditions (for laser power and detector sensitivity) be
adjusted on the brightest focus plane among sample thicknesses to be acquired to prevent
images at each focus from saturating.
8.2 Determine the range for capturing Z series images.
(1) Click the [XYZ...] button to open
the Capture Z-Series dialog box.
Note: The Z image acquisition mode is
switched according to whether or not
[Fast Piezo Z] is checked.
Checked:
Speed priority mode
Images are acquired giving a higher
priority to speed regardless of the
specified Z stroke. (This setting is
enabled only when "Nikon A1 Piezo
Z Drive" is selected as Z drive.)
Not checked:
Stroke priority mode
Images are acquired in accordance
with the specified Z stroke.
(Stroke priority mode needs a BNC
cable.)
(2) Click the
[Defined top & bottom] button.
(3) Click the [Reset] button.
(2)
(3)
Note:
(1)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available