Nikon A1 User Manual

61
Live Unmixing
(Spectral Unmixing for Live Image)
21
Chapter 21. Live Unmixing (Spectral Unmixing for Live Image)
21.1 Set the reference data to be used for spectral unmixing.
(1) Select [Image] - [Spectral Unmixing] from the menu bar to open the Spectral Unmixing Setting window.
(2) Set a spectrum to be used for unmixing from [ROIs]/[Users Defined]/[All] data of [Category] by following
the procedure for “Separating Spectral Image”.
(3) Click the [Cancel] or [Close] button to close the Spectral Unmixing Setting window.
21.2 Perform spectral unmixing for live image.
(1) Click the
[Live] button and adjust
[Laser] (laser power) and [Si HV] (detector
sensitivity) while checking the image.
Note: The Si HV setting is common to all
lasers. Make fine adjustments with the
laser power setting.
(2) Click the
[Live Unmixing] button to switch the Live image to the Unmix Live image.
(3) Click the
[Live Unmixing] button again to return to the Live image.
(2)-1
(
2
)
-2
(3)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available