Nikon A1 User Manual

72
Capturing Large Images
24
Chapter 24. Capturing Large Images
24.1 Perform Steps 4.1 to 4.7 in Chapter 4, “Capturing Color Images” to determine image
acquisition conditions.
24.2 To set Z series, set Z stack beforehand.
(1) Click the
[XYZ Time...] button to open the ND Acquisition dialog box, and then click the Z tab.
(2) Click the [Defined top & bottom] button.
(3) Click the [Live] button and move the focus knob (fine motion mode) of the microscope while
checking the image, and then click the [Top] button to determine the top position.
Note: Move the focus knob in the direction where the value of the plane in the cube increases.
(4) Click the [Live] button and move the focus knob (fine motion mode) of the microscope while
checking the image. Click the [Bottom] button to determine the bottom position.
Note: Move the focus knob in the direction where the value of the plane in the cube decreases.
(5) Determine [Step].
(6) Close the ND Acquisition dialog box.
(1)-2
(2)
(3)
(4)
(5)
(3), (4) Note
(3), (4)
(1)-1

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available