Nikon A1 User Manual

57
Separating Spectral Image
(Unmixing)
20
Chapter 20. Separating Spectral Image (Unmixing)
20.1 Click to open the spectral image.
20.2 Spectral unmixing
The following three patterns can be used in combination.
1) Spectral unmixing using spectral data in ROIs
(1) Click
at the side of the image frame
and draw ROIs on the image using tools.
Note: Specify areas that include only shades
of the same color up to the number of
pigments (2 areas or more).
(2) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(3) Select [ROIs] from the [Category] list box.
(4) Select ROIs to be used for spectral unmixing and click the [Add] button to add them to [Unmixing
Elements] (list on the right pane).
(5) Set pseudo-colors after spectral unmixing.
(6) Click the [Unmix] button to perform spectral unmixing.
(3)
(4)-1
(
4
)
-2
(5)
(6)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available