Nikon A1 - Page 58

Chapter 20. Separating Spectral Image (Unmixing) A1
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2) Spectral unmixing using reference data
(1) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(2) Select [All] from the [Category] list box.
(3) Select reagents to be used for spectral unmixing and click the [Add] button to add them to [Unmixing
Elements] (list on the right pane).
(4) Set pseudo-colors after spectral unmixing.
(5) Click the [Unmix] button to perform spectral unmixing.
3) Spectral unmixing by creating reference data
(1) Save spectral data as reference data.
Click
at the side of the image frame
and draw ROIs on the image using tools.
Note: Specify simple stain areas only.
(2)
(3)-1
(
3
)
-2
(4)
(5)