Nikon A1 User Manual

Chapter 20. Separating Spectral Image (Unmixing) A1
58
2) Spectral unmixing using reference data
(1) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(2) Select [All] from the [Category] list box.
(3) Select reagents to be used for spectral unmixing and click the [Add] button to add them to [Unmixing
Elements] (list on the right pane).
(4) Set pseudo-colors after spectral unmixing.
(5) Click the [Unmix] button to perform spectral unmixing.
3) Spectral unmixing by creating reference data
(1) Save spectral data as reference data.
Click
at the side of the image frame
and draw ROIs on the image using tools.
Note: Specify simple stain areas only.
(2)
(3)-1
(
3
)
-2
(4)
(5)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available