Nikon A1 User Manual

18
Capturing Multistained Images:
Cross Talk Reduction
(Standard Detector)
5
Chapter 5. Capturing Multistained Images: Cross Talk Reduction (Standard Detector)
5.1 Perform Steps 4.1 to 4.6 in Chapter 4, “Capturing Color Images”.
5.2 Set a channel series.
(1) Select [Custom] from the [Ch series].
(2) Click the [Custom] button to open the Line Channel Series Setup dialog box.
(3) In the Line Channel Series Setup dialog, set the order of scanning for each channel.
Setting the order of scanning arbitrarily:
Set the order of scanning channels using the channel scanning order matrix.
Using line sequence:
Select the [1->4] or [4->1] button.
Apply lasers to each scan line and start scanning.
Note: Be sure to set the TD (transmitted image) scanning order so that the scanning order comes
together with other channels because single TD scan is disabled. (Example: Laser is applied to
Ch3 and TD by second scanning.)
Fluorescence wavelength
TD
Channel
scanning order matrix
Scanning order
Line sequence
Channel names

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available