Nikon A1 - Page 60

Chapter 20. Separating Spectral Image (Unmixing) A1
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(5) Select [Image] - [Spectral Unmixing Setting] from the menu bar.
(6) Select [User Defined] from the [Category] list box.
(7) Select reagents (spectral data saved in step 4) to be used for spectral unmixing and click the [Add] button
to add them to [Unmixing Elements] (list on the right pane).
(8) Set pseudo-colors after spectral unmixing.
(9) Click the [Unmix] button to perform spectral unmixing.
How to use Spectrum Profile
Displays the Y axis of graph as
relative values.
Displays the Y axis of graph as
brightness values.
Inserts a spectrum graph originated by
the fluorescence manufacturer
as reference of analysis.
(6)
(7)-1
(7)-2
(8)
(9)