Nikon A1 - Page 28

Chapter 8. Capturing Z Series Images A1
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(4) Click the [Live] button and move the focus knob (fine motion mode) of the microscope while
checking the image, and then click the [Top] button to determine the top position.
Note: Move the focus knob in the direction where the value of the plane in the cube increases.
(5) Click the [Live] button and move the focus knob (fine motion mode) of the microscope while
checking the image. Click the [Bottom] button to determine the bottom position.
Note: Move the focus knob in the direction where the value of the plane in the cube decreases.
(6) Determine [Step].
(7) Select “Ti ZDrive” for [Z Device].
(8) Check the Save to File checkbox as needed, and acquire images while saving them.
Note: Images are saved in nd2 file format.
(4), (5)
(4)
(5)
(4), (5) Note
(8)
(6)
(7)