62 . Lambda XLS/XLS+ User's Guide
Kinetics
Kinetics studies, where the change in absorbance needs to be followed as a function of time
at a fixed wavelength, can readily be performed using the Kinetics method.
Reagent test kits are routinely used for the enzymatic determination of compounds in food,
beverage and clinical laboratories by measuring NAD/NADH conversion at 340 nm. The
change in absorbance over a specified time period can be used to provide useful information
when an appropriate factor, defined in the reagent-kit protocol, is applied. Reaction rate and
enzyme activity can be calculated if the factor used takes account of the absorbance
difference per unit time, as opposed to only the absorbance difference.
For this reason, the change in absorbance per minute (∆A/min), concentration (∆A/min x
factor) and correlation coefficient (calculated from a best fit of the data points) are displayed.
They may not be relevant for simple kinetics experiments.
The procedure to define a new method is as follows:
1. From the home page, press 1 to open the Standard Methods folder.
The Standard Methods screen is displayed.
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