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PerkinElmer Lambda XLS - Absorbance Ratio Calculations

PerkinElmer Lambda XLS
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Instrument Methods . 67
Absorbance Ratio
The Absorbance Ratio method makes simple absorbance ratio measurements on samples, by
measuring the amount of light that has passed through a sample relative to a blank (this can
be air) at two wavelengths.
The procedure is as follows:
1. From the home page, press 1 to open the Standard Methods folder.
The Standard Methods screen is displayed.
2. Press 7 to select the Absorbance Ratio method.
The Absorbance Ratio Wavelengths screen is displayed.
3. Enter Wavelength 1 by using the keypad numbers or the left and right arrows, and
then press the down arrow.
4. Enter Wavelength 2 as above and then press the down arrow.
5. Select whether a Background correction is applied to both wavelengths 1 and 2 using
the left and right arrows.

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