PerkinElmer Spectrum 100 Series - Principles of Use

100 . Spectrum 100 Series User’s Guide
Performance Validation Kits
The Spectrum 100N is supplied with Performance Validation kit L1180479, which contains
a 35 µm polystyrene reference sample and an NG11 Schott Glass reference sample.
For the Spectrum 100, Performance Validation kit L1365335 is available. This kit contains
a 1.2 mm polystyrene reference sample. The Performance Validation procedure is similar
to that described here, but the kit includes IPV Kit User’s Guide 09934028.
Principles of Use
The Food and Drug Administration (FDA), the United Kingdom Accreditation Service
(UKAS) and the Good Laboratory Practice (GLP) regulations, all require that calibration of
instrument accuracy can be traced to a certified reference material (CRM).
Instrument Performance Validation (IPV) enables you to routinely generate a record of
wavenumber calibration that is traceable to a National Physical Laboratory (NPL) CRM.
Using the NIR IPV certificate printing utility, you produce a certificate comparing the
result for a polystyrene Traceable Reference Material (TRM), and your internal
polystyrene Automatic Precision Validator (APV) against a certified polystyrene standard
(serial number: JR95), measured by the NPL, Teddington, TW1 0LW, UK.
The NPL certificate of measurement provides wavenumber values for two peaks,
5669.3 cm
-1
and 4571.6 cm
-1
, for resolutions of 4 cm
-1
or better. The polystyrene TRM
supplied with your instrument has been calibrated against this CRM, and a Certificate of
Calibration printed at the factory is included with it. This certificate provides a record of
calibration and traceability for the TRM to the NPL CRM.
The polystyrene in the APV is calibrated by the procedure described in the following
pages. A certificate of Performance Validation will be printed which details the calibration
of the polystyrene in the APV against the TRM.
The certification procedure also validates the internal APV used by Spectrum 100N for
Ready-for-Work checks. For more information see the Spectrum on-screen help which
can be accessed by selecting Contents and Index from the Help menu in Spectrum.
Limitations of traceable wavenumber calibration
Certified values for the peak positions used have quoted uncertainties of
±1.5 cm
-1
for the peak at 5669.3 cm
-1
and ±1.0 cm
-1
for the peak at 4571.6 cm
-1
. We
suggest that the uncertainty associated with routine measurement of the two
recommended peaks, in the spectrum of a traceable polystyrene sample, is realistically
set at these limits. This level of precision is satisfactory for general analytical applications
involving solid and liquid samples.