2.25
Collection of blood using capillary blood collection
tubes
The blood is collected into a capillary blood collection tube. Please
ensure that the collection tube is coated with an anticoagulant suit-
able for the test selected. For full details of suitable anticoagulants for
the various parameters please consult the package insert accom-
panying the respective Reflotron test. Your supplier will inform you
where to obtain tubes suitable for the collection or storage of capil-
lary blood. These tubes are coated either with Li-heparin or EDTA and
are available in different sizes (for example, Microvette
®
CB 300 or
Microtainer
®
).
The tube should be filled up to the maximum mark. This is facilitated
by choosing an appropriately-sized tube. When blood collection has
been completed the tube is sealed and the blood and anticoagulant
immediately mixed thoroughly by inverting the tube or using an auto-
matic mixer.
Pipetting with the Reflotron Pipette
The specimen can be pipetted with commercially available pipettes
which deliver a volume of 30 µl. Please follow the manufacturer’s
instructions for the pipette used.
The Reflotron Pipette allows blood to be aspirated from blood col-
lection tubes without air bubbles. Should it nevertheless become
necessary to discard a sample because of air bubbles, a new pipette
tip must be used. Wipe off any sample material adhering to the out-
side of the pipette tip. If the pipette becomes contaminated while
drawing up the sample, it must be disinfected.
For more details on the use of Reflotron Pipette R see “Working with
Reflotron tests and blood, serum or plasma” in Section 2.
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