2.28
Obtaining capillary plasma
Capillary plasma may be obtained using commercially available ca-
pillary blood collection tubes (microcollection tubes) and an appropri-
ate centrifuge. In this case the manufacturer’s specifications on the
proper use of the collection tubes should be observed.
After preparing the puncture site (see above) and wiping off the first
drop, collect the capillary blood into a collection tube containing an
anticoagulant that is suitable for the requested test.
Fill the tube up to the upper mark (select a proper size according to
the sample volume required). Avoid pressing or squeezing the punc-
ture site, as this may lead to hemolysis and could influence the test
result.
Having filled the collection tube, seal it and immediately mix the
blood with the anticoagulant by repeatedly inverting the tube or using
a mixer.
To obtain plasma, insert the collection tube and a counterbalance
into a rotor suitable for the kind of tube used and centrifuge. If nec-
essary, use a suitable insert for the centrifuge.
Observe the instructions given by the manufacturer of the centrifuge
and the respective safety warnings.
Collecting the specimen
Centrifuging the specimen