tecan ls series - Page 110

6. LSServer, Scanner Shell and Download
6-80 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5 2004-11
2. Channel 2 tab
Before calculating crosstalk correction a so-called K-factor must be
determined. This is done during a measurement without taking into account
crosstalk correction. Adjusted values in this measurement session must be
entered in
Channel 2 tab.
The
Channel 2 tab contains the following elements:
Dye 2
edit box
In the
Dye 2 edit box the description of used dye must be
entered.
K2
edit box
In the
K2 edit box the correction factor for contribution of dye
with longer emission wavelength into the detection channel
of dye with shorter emission wavelength must be entered.
The
K2 default value is set to 0. This means no crosstalk
correction will be done, no other parameters (Dye, gain)
must be specified.
Gain 1
edit box
In the
Gain 1 edit box PMT Gain channel 1 must be entered.
Gain 2
edit box
In the
Gain 2 edit box PMT Gain channel 2 must be entered.
Next part of
Crosstalk dialog box contains the following elements:
Channel 1 Laser
combo box
In
Channel 1 Laser combo box all lasers found in current
connected instrument are displayed. The one used for
crosstalk correction must be selected.
Channel 2 Laser
combo box
In
Channel 2 Laser combo box all lasers found in current
connected instrument are displayed. The one used for
crosstalk correction must be selected.
Channel 1 Filter
combo box
In
Channel 1 Filter combo box all filters found in filter slide,
loaded in slot 1 of current connected instrument, are
displayed. The one used for crosstalk correction must be
selected.
Channel 2 Filter
combo box
In
Channel 2 Filter combo box all filters found in filter slide,
loaded in slot 2 of current connected instrument, are
displayed. The one used for crosstalk correction must be
selected.
Date static box The Date static box shows the current date.
Format = DD.MM.YY
Name edit box In the Name edit box the user can type in his name or initials
or some other comment.