196 Running Gradients
To begin a run with an external non-PowerLine autoinjector:
1. Load the sample vials into the autoinjector.
2. Program and start the autoinjector. See the autoinjector manual.
3. When the autoinjector makes the injection, it sends a contact closure signal to the
Inject input terminal on the rear panel of the controller.
Using the Start Run Screen Key with a Non-PowerLine Autoinjector
For methods development and for runs that are not time-sensitive, you may also use the
Start Run screen key to start a run with a non-PowerLine autoinjector.
To start a run with an autoinjector using the Start Run key:
1. Load the sample vials into the autoinjector.
2. Program and start the autoinjector. See the autoinjector manual.
3. Press the Start Run key as soon as possible after the injection occurs.
Note: An Autostart gradient can override a gradient that you started with the Start Run
screen key.
Starting the Run-Time Clock
When the controller receives an Inject signal from an injector, the run-time clock begins
counting at 0.00. The system does not accept another Inject signal until the run completes
or you abort the run.
Completing the Gradient
The Operate Gradient screen displays the current conditions during the run. The gradient
and event tables continue to run until you manually stop them by pressing the Initial
screen key, making another injection, or aborting the run.
The external events remain in their set conditions when each analysis completes. They do
not automatically reset to Off. Events that need to be turned on at a specific time in an
analysis must be programmed to turn off before the start of the next injection.
The run-time clock continues to run after each analysis completes and resets to 0.00 only
with a new injection. Events or gradient segments programmed to execute after the run
time for each injection still occur.
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