Overview 1
Overview
Column temperature variations can shift peak retention times and alter peak
shapes, increasing the difficulty of achieving precise results. The ACQUITY
UPLC
®
column compartments help to ensure precise, reproducible
separations by controlling the column temperature.
Note: If you are using an ACQUITY UPLC system with two dimensional
applications, refer to the ACQUITY UPLC Systems with 2D Technology
Capabilities Guide.
Column compartment types
The following column compartments are available for use with ACQUITY
UPLC systems:
• Column heater (CH-A)
• 30-cm column heater (CH-30A)
• Column manager (CM-A)
• Auxiliary column manager (CM-Aux)
• 30-cm column heater/cooler (ACQUITY UPLC 30-cm CHC)
Each of these column compartments is also available in a bio-compatible
version.
Column heater (CH-A)
A standard component of the basic ACQUITY UPLC system, the column
heater heats the column compartment to any temperature, from 20ºC to
90 ºC, that is at least 5 ºC above the ambient temperature. A standard, active
preheater heats the incoming solvent before it enters the column.
Additionally, you can install a supplementary column stabilizer.
The column heater can accommodate UPLC
®
columns with dimensions of as
much as 4.6 mm, internal diameter (ID), and 150-mm long.
To reduce dispersion associated with dead volume, and minimize the length of
tubing between system instruments, the column compartment is directly
above the sample manager and connected to the optical detector (on top of the
column heater). For MS applications, you can mount the column
compartment, on a bracket to the right-hand side of the system stack,
reducing the distance to the mass spectrometer’s inlet probe.
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