2.1.1 Follow these general recommendations when performing a
UPLC analysis
Select appropriate solutions
• Use high-quality solvents, buffers, and additives (HPLC or MS grade).
• Keep concentrated stock solutions, to use when preparing working solutions.
• Start gradients that include an organic component (0.1%, for example) to provide more
consistent and predictable gradient formation than when you start with 0% organic.
• Ensure that gradients include an organic component (0.1%, for example) to start, to provide
more consistent and predictable.
Set up the system properly
• When installing or removing a column, always hold the active preheater’s reusable
compression fitting in place. Rotate the column or optional in-line filter to install or remove it.
• Always use solvent filters on tubing lines in solvent bottles.
• Use the Load Ahead option when you desire a shorter cycle time.
Prime properly
• Prime solvent lines during system start-up.
• Keep the seal wash line and all solvent lines primed.
Manage waste properly
• Do not block the degasser vent line; trim the tubing, if necessary.
• Do not submerge the waste or degasser vent lines in liquid. (See the solvent manager
overview and maintenance guide for details on how to route the tubing.)
• Monitor the waste level, to ensure that it is never too high.
Use care when using buffers
• Do not use buffers in the wash solvent line.
• Do not top off buffers, which can promote microbial growth.
• Filter buffers with a 0.2-µm filter membrane.
• Do not leave buffers stored in the system.
August 8, 2016, 715005049 Rev. C
Page 27
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