Waters ACQUITY - Sample Manager - Flow through Needle Recommendations; Common Solvent Properties

B.13 Sample manager - flow through needle
recommendations
• Do not use concentrations of THF or hexane greater than 10% as purge solvent.
• Typical organic sample diluents such as dimethylsolfoxide (DMSO) and dimethylformamide
(DMF) are supported.
Notice:
• Do not leave buffers stored in the system.
• Flush all flow paths, including the needle wash, with plenty of non-buffered solvent
before shutting down the system.
• For extended shutdown periods (longer than 24 hours), use 10% to 20% methanol
in water.
• When using a buffered wash solvent, prime it for a minimum of 30 sec.
• Use of buffers can cause salt build-up on the needle and wash port, which can
require periodic cleaning.
B.14 Common solvent properties
The following table lists the properties for some common chromatographic solvents.
Table B–1: Properties of common solvents
Solvent Vapor pressure mm Hg (Torr) Boiling point
(°C)
Flash point
(°C)
Acetone 184.5 at 20 °C 56.29 -20
Acetonitrile 88.8 at 25 °C 81.6 6
n-butyl acetate 7.8 at 20 °C 126.11 22
n-butyl alcohol 4.4 at 20 °C 117.5 37
n-butyl chloride 80.1 at 20 °C 78.44 -9
Chlorobenzene 8.8 at 20 °C 131.69 28
Chloroform 158.4 at 20 °C 61.15
Cyclohexane 77.5 at 20 °C 80.72 -20
Cyclopentane 400 at 20 °C 49.26 -7
o-Dichlorobenzene 1.2 at 20 °C 180.48 66
Dichloromethane 350 at 20 °C 39.75
Dimethyl acetamide 1.3 at 25 °C 166.1 70
August 8, 2016, 715005049 Rev. C
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