Consider solvent polarity when you change typical reversed-phase solvents. Flush the system
with a solvent of intermediate polarity, like isopropanol, before introducing nonpolar solvents like
THF or hexane.
B.6
Hexane/THF compatibility kit
The ACQUITY UPLC System Hexane/THF Compatibility Kit (contact Waters for part number) can
be installed in ACQUITY UPLC systems with closed waste management. It is designed for users
that need to run their systems with hexane or THF at high concentrations and high pressure and
is recommended for many ELS detector-based applications where THF is used in the mobile
phase, at high concentrations.
B.7 Additives/modifiers
• 0.1% ethylene diaminetetraacetic acid (EDTA)
• 0.1% heptafluorobutyric acid
• 0.1% triethyl amine (TEA)
• 0.1% trifluoroacetic acid (TFA)
• 0.2% formic acid
• 0.3% acetic acid
• 10 mM ammonium bicarbonate
• 10 mM phosphate buffer
• 50 mM ammonium acetate
• 50 mM ammonium hydroxide
B.8
Sample diluents
• Acetonitrile
• Acetonitrile/water mixtures
• Chloroform
• Dimethylformamide (DMF)
• Dimethyl sulfoxide (DMSO)
• Isooctane
• Isopropanol
August 8, 2016, 715005049 Rev. C
Page 89
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