Zeiss Axiovert 200 - Page 102

OPERATION
Carl Zeiss Illumination and contrasting techniques Axiovert 200
3-42 B 40-080 e 03/01
• Switch on the NHBO 103 fluorescence illuminator (3-24/1) via the power supply and allow it to heat
to operating temperature for approx. 15 minutes.
• Select the FL reflector module in the reflector turret containing the required fluorescence filter
combination (depending on the excitation) and switch it on.
• Press the FL on/off key (3-24/6) to open the fluorescence shutter. The opening of the shutter is
confirmed by a beep.
For the epi-fluorescence technique, one slider each with iris stop is used as aperture and luminous-field
diaphragm. However, since the aperture diaphragm is not visible in reflected light, the aperture
diaphragm slider must first be centered in the compartment of the luminous-field diaphragm and then
be inserted in the compartment for the aperture diaphragm.
The centering for the aperture diaphragm and the luminous-field diaphragm is performed in the same
way:
• Insert an iris stop slider into the compartment (3-24/3) for the luminous-field diaphragm until stop.
• Use the lever to close the diaphragm until it becomes visible in the field of view.
• Then center the diaphragm using the two adjusting screws in the slider (with SW 3 mm ball-headed
screwdriver). Open the diaphragm until the entire field is uncovered.
• Push the slider centered in this way into the compartment for the aperture diaphragm (3-24/2) until
stop.
• Insert an additional iris stop slider into the compartment (3-24/3) for the luminous-field diaphragm.
• Close the luminous-field diaphragm until it becomes visible in the field of view.
• Use the two centering screws to center the luminous-field diaphragm in the edge of the field of view.
• Either open the luminous-field diaphragm until it just disappears behind the edge of the field of view
or, if there is a risk of specimen bleaching, reduce it until it is visible in the field of view.
• Finally, refocus on the specimen and optimize the collector position of the fluorescence illuminator.
For this purpose, use the knurled knob on the fluorescence illuminator to set the collector in such a
way that illumination of the field of view provided by the short-wave excitation reflector module is as
homogeneous as possible. Correction of the collector position is not required when modules with
long-wave excitation are used.