OPERATION
Axiovert 200 Illumination and contrasting techniques Carl Zeiss
B 40-080 e 03/01 3-29
3.3.1.3 Transmitted-light brightfield setting according to KÖHLER on the Axiovert 200
(manual)
− Put the Axiovert 200 (manual) in operation as described in chapter 2.
− Switch on the Axiovert 200 (manual).
• Select the objective with the lowest magnification (e.g. 10x objective with the yellow ring) on the
nosepiece (3-16/2) and ensure the correct stop position.
• Set factor 1x on the setting wheel (3-16/4) of the Optovar turret; ensure the correct stop position.
• Open the luminous-field diaphragm completely by pulling the lever (3-16/16) on the carrier for
transmitted-light illumination to the front until stop.
• Open the aperture diaphragm completely by turning the setting wheel (3-16/20) on the condenser to
the front until stop.
• Turn the setting ring (3-16/19) to move the condenser turret in position H for brightfield (if not
available, to position DIC).
• Turn the setting ring to move the reflector turret (3-16/5, if available) in a position without filter
combination and ensure the correct stop position.
• If required, remove analyzer slider from the compartment (3-16/3) or switch to free light path; ensure
the correct stop position.
• Turn setting wheel for Sideport right / Sideport left / vis (3-16/22) to position 100 % vis (visual).
• Turn setting knob for Frontport / Baseport / vis (3-16/23) to position 100 % vis.
• Set beam splitting ratio to 100 % vis (3-16/10) on the binocular (photo)tube. Switch off the Bertrand
lens (if available). Move combined rotary / slider knob (3-16/9) to position 100 % vis.
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